101 128
B
% of GFP
+
cells
GFP
SSC-A
A
Relative mRNA
expression
INS-GFP
+
cells
D
F
c-peptide (ng /1k cells)
30.9
30.2
29.6
29.9
INS
NKX2.2
DAPI
INS
NKX6.1
DAPI
INS
PDX1
DAPI
E
INS
NKX6.1 glucagon
human beta cells
CDKAL1
GAPDH
wt
CDKAL1
-/-
#1 #2 #1 #2
KCNQ1
GAPDH
wt
KCNQ1
-/-
KCNJ11
GAPDH
wt
KCNJ11
-/-
C
wt -#1
wt -#2
CDKAL1
-/-
-
#1
CDKAL1
-/-
-
#2
KCNQ1
-/-
-
#1
KCNQ1
-/-
-
#2
KCNJ11
-/-
-
#1
KCNJ11
-/-
-
#2
28.7
27.5
29.1
30.8
wt
CDKAL1
-/-
KCNQ1
-/-
KCNJ11
-/-
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
#1 #2 #1 #2 #1 #2 #1 #2
wt
CDKAL1
-/-
KCNQ1
-/-
KCNJ11
-/-
0
5
10
15
20
25
30
35
#1 #2 #1 #2 #1 #2 #1 #2
human
beta cells
#1 #2 #1 #2
#1 #2 #1 #2
wt
CDKAL1
-/-
KCNQ1
-/-
KCNJ11
-/-
25.7 24.0 23.0 24.8 24.9 23.2 25.1 25.3
20.0 21.6 22.9 23.3 20.1 21.2 20.7 22.0
37 kDa
65 kDa
37 kDa
74 kDa
37 kDa
44 kDa
#1
#2
wt -#1
wt -#2
CDKAL1
-/-
-
#1
CDKAL1
-/-
-
#2
KCNQ1
-/-
-
#1
KCNQ1
-/-
-
#2
KCNJ11
-/-
-
#1
KCNJ11
-/-
-
#2
0
0.2
0.4
0.6
0.8
1
1.2
1.4
CDKAL1 KCNQ1 KCNJ11
n.s. n.s. n.s.
(legend on next page)
Cell Stem Cell
19
, 326–340, September 1, 2016
327