CRISPR Gene Editing in Stem Cells

38 128

on-target activities comparable with wild-type Cas9 [113]. Combinations of these methods
could provide a route to its ultimate use for gene therapy.
As a powerful, yet versatile, gene-editing and regulation tool, CRISPR[5_TD$DIFF]/Cas9 technology is
already accelerating both research and therapeutics. We believe that its broad applications
in genomics research and cell biology research will greatly advance our knowledge of both basic
biology and diseases in the years to come.
[6_TD$DIFF]Acknowledgments
F.W. acknowledges support from the Center of Synthetic Biology and Shanghai Institute of Rheumatology in Renji Hospital
affiliated to Shanghai Jiaotong University School of Medicine, National Natural Science Foundation of China (No.
81502233), the Science and Technology Commission of Shanghai Municipality (No. 134119a8100) and the Young
Scientifi c Research Project of Shanghai Municipal Health Bureau (No. 20134Y176). L.S.Q. acknowledges support from
the NIH Offi ce of the Director (OD), National Institute of Dental & Craniofacial Research (NIDCR), and NIH Director's Early
Independence Award DP5 OD017887.
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886 Trends in Cell Biology, November 2016, Vol. 26, No. 11